Detection and Assay of Mitochondrial Aspartate Aminotransferase in Serum
نویسندگان
چکیده
منابع مشابه
Detection and assay of mitochondrial aspartate aminotransferase in serum.
Aspartate aminotransfcrase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2. 6.1.1) comprises two isoen&ymcs. One is anionic, is associated with the soluble fraction of the cell and ordinarily forms the bulk of the scrum activity. The other is cationic, and is associated with the mitochondria (1—7). Each may in turn be subdivisible (8—10), but it is unlikely that this will influence the pres...
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The activity of aspartate aminotransferase (AST) in human serum has been widely determined as a diagnostic aid in liver disease. In this study, the effect of aluminium on AST isoenzymes in relation to aluminium intoxified patients has been investigated. Using gel filtration chromatography technique with Sephacryl S-300, mitochondrial aminotransferase (m-AST) and cytosolic aminotransferase (c-AS...
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The isoenzymes of aspartate transaminase differ in their kinetic properties in that the cytoplasmic isoenzyme is more readily inhibited by adipate and by 2-oxoglutarate (substrate) at low pH. A differential kinetic assay based on this phenomenon has been optimised for use in assays of serum samples. The new method agrees well with an immune absorption procedure. Methods based on chromatographic...
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Effects of serum and protein on assays measuring aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) were examined. Increasing amounts of normal human serum or human serum albumin in samples with fixed amounts of purified enzyme decrease absorbance values and thus distort the results obtained in an assay in which diazonium-salt chromogenic reagent is used. Seru...
متن کاملchanges in the level of mitochondrial and cytosolic aspartate aminotransferase activities in aluminium intoxified rat
the activity of aspartate aminotransferase (ast) in human serum has been widely determined as a diagnostic aid in liver disease. in this study, the effect of aluminium on ast isoenzymes in relation to aluminium intoxified patients has been investigated. using gel filtration chromatography technique with sephacryl s-300, mitochondrial aminotransferase (m-ast) and cytosolic aminotransferase (c-as...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1968
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1968.6.5.431